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id7000 software  (Sony Biotechnology)


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    Structured Review

    Sony Biotechnology id7000 software
    Configuration of <t>ID7000</t> The SONY ID7000 is equipped with five lasers (355□ nm, 405 □nm, 488 □nm, 561 □nm, and 637□ nm) and 149 detectors, enabling detection of the full fluorochrome emission spectrum.
    Id7000 Software, supplied by Sony Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 788 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/id7000+software/ID7000+Spectral+Cell+Analyzer/bio_rxiv__64898__2026__10__13__758716-91-9-11
    Average 99 stars, based on 788 article reviews
    id7000 software - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay"

    Article Title: A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay

    Journal: bioRxiv

    doi: 10.64898/2026.10.13.758716

    Configuration of ID7000 The SONY ID7000 is equipped with five lasers (355□ nm, 405 □nm, 488 □nm, 561 □nm, and 637□ nm) and 149 detectors, enabling detection of the full fluorochrome emission spectrum.
    Figure Legend Snippet: Configuration of ID7000 The SONY ID7000 is equipped with five lasers (355□ nm, 405 □nm, 488 □nm, 561 □nm, and 637□ nm) and 149 detectors, enabling detection of the full fluorochrome emission spectrum.

    Techniques Used:


    Figure Legend Snippet: Instrument configuration for the SONY 5 laser ID7000. Sony ID7000 flow cytometer laser detection channels and fluorescent antibodies. The antibodies and fluorophores used in this panel are indicated on the corresponding detectors.

    Techniques Used: Flow Cytometry

    Unmixing and Adjustments: N×N plots viewed with the ID7000™ Unmixing Viewer. Unmixing was performed on the live cell population. Unmixed data with B220-BUV496 depicted as an example on the x-axis and all other fluorochromes plotted on the y-axis. Examples of unmixing errors and corrected unmixing results are indicated by red boxes
    Figure Legend Snippet: Unmixing and Adjustments: N×N plots viewed with the ID7000™ Unmixing Viewer. Unmixing was performed on the live cell population. Unmixed data with B220-BUV496 depicted as an example on the x-axis and all other fluorochromes plotted on the y-axis. Examples of unmixing errors and corrected unmixing results are indicated by red boxes

    Techniques Used:

    Related Articles

    Software:

    Article Title: Translational toolkit for reproducible, cross-study profiling of human ageing hallmarks in human blood and tissue
    Article Snippet: Finally, the cells were resuspended in 200 μl of MACS buffer and were run on the SONY ID7000 spectral flow cytometer (SONY Biotechnology, ID7000TM Spectral Cell Analyzer). .. Samples were analysed using the ID7000 software (SONY Biotechnology, ID7000TM Software System). ..

    Article Title: SARS-CoV-2 reinfection broadens the antibody responses and promotes the phenotypic differentiation of virus-specific memory T cells in adolescents.
    Article Snippet: .. All samples were acquired on an ID7000TM Cell Analyzer (Sony Biotechnology) and analyzed using the ID7000 Software (https://www. sonybiotechnology.com/us/instruments/id7000‐spectral‐cell‐ analyzer/software/). ..

    Article Title: Natural senolytic activity of Rhodiola rosea extract alleviates age-associated phenotypes via paraptosis
    Article Snippet: .. ID7000 software (version 1.1.0.11041) , Sony, Japan , N/A. .. Living Image software (version 4.5.5) , Perkin Elmer, USA , N/A.

    Article Title: Cirrhosis-associated immune dysfunction presents with preserved circulating lymphocyte function and altered intestinal intraepithelial lymphocyte profile
    Article Snippet: Data were acquired on a Sony ID7000 spectral cell analyzer, and fluorescence was collected as full emission spectra across all detectors for each laser line. .. Single-stained controls (PBMC spiked with tissue cells or beads) for each fluorochrome and an unstained control were used to generate reference spectra, which were then applied for spectral unmixing using the instrument’s weighted least-squares algorithm implemented in the ID7000 software (Sony Biotechnology). ..

    Article Title: A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay
    Article Snippet: .. All flow cytometry data was acquired or analyzed using ID7000 software (Sony Biotechnology). ..

    Article Title: Establishment of a humanized patient-derived xenograft mouse model of high-grade serous ovarian cancer for preclinical evaluation of combination immunotherapy.
    Article Snippet: Pelleted cells were incubated in a solution of human Fc-receptor blocking agent (cat. no.: 130-059-901; Miltenyi Biotec,) and anti-mouse-CD16/CD32 monoclonal antibody (cat. no.: 16-0161-82; Thermo Fisher Scientific), and then stained with the antibody mix defined in Table S1. .. Stained cells were washed twice with a mix of 2% V/V FBS in PBS and acquired on an ID7000 Spectral Cell Analyzer (LE-ID7000C; Sony Biotechnology) equipped with ID7000 Software (v2.0.2.17121; Sony Biotechnology). .. Single-cell data were processed using FlowJo software (v10.10.0; Becton, Dickinson and Company) (Fig. S4).

    Article Title: Challenges of monocyte HLA-DR targeted immunomodulation in sepsis—a prospective observational cohort study
    Article Snippet: .. Cells were acquired using an ID7000 spectral cell analyzer (Sony Biotechnology Inc., Weybridge, UK) running the ID7000 software (version 1.2, Sony). .. Alignment check beads (Sony) were used prior to each experiment.

    Control:

    Article Title: Cirrhosis-associated immune dysfunction presents with preserved circulating lymphocyte function and altered intestinal intraepithelial lymphocyte profile
    Article Snippet: Data were acquired on a Sony ID7000 spectral cell analyzer, and fluorescence was collected as full emission spectra across all detectors for each laser line. .. Single-stained controls (PBMC spiked with tissue cells or beads) for each fluorochrome and an unstained control were used to generate reference spectra, which were then applied for spectral unmixing using the instrument’s weighted least-squares algorithm implemented in the ID7000 software (Sony Biotechnology). ..

    Flow Cytometry:

    Article Title: A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay
    Article Snippet: .. All flow cytometry data was acquired or analyzed using ID7000 software (Sony Biotechnology). ..

    Staining:

    Article Title: Establishment of a humanized patient-derived xenograft mouse model of high-grade serous ovarian cancer for preclinical evaluation of combination immunotherapy.
    Article Snippet: Pelleted cells were incubated in a solution of human Fc-receptor blocking agent (cat. no.: 130-059-901; Miltenyi Biotec,) and anti-mouse-CD16/CD32 monoclonal antibody (cat. no.: 16-0161-82; Thermo Fisher Scientific), and then stained with the antibody mix defined in Table S1. .. Stained cells were washed twice with a mix of 2% V/V FBS in PBS and acquired on an ID7000 Spectral Cell Analyzer (LE-ID7000C; Sony Biotechnology) equipped with ID7000 Software (v2.0.2.17121; Sony Biotechnology). .. Single-cell data were processed using FlowJo software (v10.10.0; Becton, Dickinson and Company) (Fig. S4).



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    Image Search Results


    Configuration of ID7000 The SONY ID7000 is equipped with five lasers (355□ nm, 405 □nm, 488 □nm, 561 □nm, and 637□ nm) and 149 detectors, enabling detection of the full fluorochrome emission spectrum.

    Journal: bioRxiv

    Article Title: A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay

    doi: 10.64898/2026.10.13.758716

    Figure Lengend Snippet: Configuration of ID7000 The SONY ID7000 is equipped with five lasers (355□ nm, 405 □nm, 488 □nm, 561 □nm, and 637□ nm) and 149 detectors, enabling detection of the full fluorochrome emission spectrum.

    Article Snippet: All flow cytometry data was acquired or analyzed using ID7000 software (Sony Biotechnology).

    Techniques:

    Journal: bioRxiv

    Article Title: A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay

    doi: 10.64898/2026.10.13.758716

    Figure Lengend Snippet: Instrument configuration for the SONY 5 laser ID7000. Sony ID7000 flow cytometer laser detection channels and fluorescent antibodies. The antibodies and fluorophores used in this panel are indicated on the corresponding detectors.

    Article Snippet: All flow cytometry data was acquired or analyzed using ID7000 software (Sony Biotechnology).

    Techniques: Flow Cytometry

    Unmixing and Adjustments: N×N plots viewed with the ID7000™ Unmixing Viewer. Unmixing was performed on the live cell population. Unmixed data with B220-BUV496 depicted as an example on the x-axis and all other fluorochromes plotted on the y-axis. Examples of unmixing errors and corrected unmixing results are indicated by red boxes

    Journal: bioRxiv

    Article Title: A 21□Color Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Rat Tissues and a Companion 9□Color T□Cell Functional Assay

    doi: 10.64898/2026.10.13.758716

    Figure Lengend Snippet: Unmixing and Adjustments: N×N plots viewed with the ID7000™ Unmixing Viewer. Unmixing was performed on the live cell population. Unmixed data with B220-BUV496 depicted as an example on the x-axis and all other fluorochromes plotted on the y-axis. Examples of unmixing errors and corrected unmixing results are indicated by red boxes

    Article Snippet: All flow cytometry data was acquired or analyzed using ID7000 software (Sony Biotechnology).

    Techniques:

    Optimization of a 45-color spectral panel (A) “Spectral Viewer” overview of all 45 fluorescence spectra acquired using a six-laser–configured ID7000 (320, 355, 405, 488, 561, and 637 nm) with 182 detector channels. (B) Spillover spreading matrix calculated in FlowJo V10.10.0 from single stains. (C) Titration of antibodies in human PBMCs. The viability dye Zombie NIR and CD73-biotin/Qdot 525 streptavidin were independently optimized (see for final volumes).

    Journal: STAR Protocols

    Article Title: Protocol for generating high-quality, 45-color spectral flow cytometry data for unsupervised clustering to investigate aging in human PBMCs

    doi: 10.1016/j.xpro.2026.104380

    Figure Lengend Snippet: Optimization of a 45-color spectral panel (A) “Spectral Viewer” overview of all 45 fluorescence spectra acquired using a six-laser–configured ID7000 (320, 355, 405, 488, 561, and 637 nm) with 182 detector channels. (B) Spillover spreading matrix calculated in FlowJo V10.10.0 from single stains. (C) Titration of antibodies in human PBMCs. The viability dye Zombie NIR and CD73-biotin/Qdot 525 streptavidin were independently optimized (see for final volumes).

    Article Snippet: ID7000 Software V2.1 , Sony , https://www.sonybiotechnology.com/instruments/id7000-spectral-cell-analyzer/software.

    Techniques: Fluorescence, Titration

    Autofluorescence (AF) unmixing workflow for the 45-color panel (A) Ribbon plot showing the fluorescence spectrum of unstained PBMCs. Autofluorescence is especially excited by the 320 nm and 355 nm lasers. (B) In the ID7000 Software, the “Autofluorescence Finder” tool is employed to identify and unmix the AF of two distinct populations within the AF root gate, excited by the 320 nm and 355 nm lasers. (C) AF-A and AF-B are highlighted in the “Spectral Reference Viewer” among the 45-color + 2 AF spectra. The fluorochromes with the greatest AF overlap are BUV496 (turquoise) and SparkUV 387 (purple). (D) AF unmixing improves population separation by extracting AF contributions to the negative population for CD28-BUV496 and CD14-SparkUV 387.

    Journal: STAR Protocols

    Article Title: Protocol for generating high-quality, 45-color spectral flow cytometry data for unsupervised clustering to investigate aging in human PBMCs

    doi: 10.1016/j.xpro.2026.104380

    Figure Lengend Snippet: Autofluorescence (AF) unmixing workflow for the 45-color panel (A) Ribbon plot showing the fluorescence spectrum of unstained PBMCs. Autofluorescence is especially excited by the 320 nm and 355 nm lasers. (B) In the ID7000 Software, the “Autofluorescence Finder” tool is employed to identify and unmix the AF of two distinct populations within the AF root gate, excited by the 320 nm and 355 nm lasers. (C) AF-A and AF-B are highlighted in the “Spectral Reference Viewer” among the 45-color + 2 AF spectra. The fluorochromes with the greatest AF overlap are BUV496 (turquoise) and SparkUV 387 (purple). (D) AF unmixing improves population separation by extracting AF contributions to the negative population for CD28-BUV496 and CD14-SparkUV 387.

    Article Snippet: ID7000 Software V2.1 , Sony , https://www.sonybiotechnology.com/instruments/id7000-spectral-cell-analyzer/software.

    Techniques: Fluorescence, Software