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id7000 software  (Sony Biotechnology)


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    Sony Biotechnology id7000 software
    Id7000 Software, supplied by Sony Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 719 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/id7000+software/pmc13101718-79-0-5?v=Sony+Biotechnology
    Average 99 stars, based on 719 article reviews
    id7000 software - by Bioz Stars, 2026-08
    99/100 stars

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    Optimization of a 45-color spectral panel (A) “Spectral Viewer” overview of all 45 fluorescence spectra acquired using a six-laser–configured ID7000 (320, 355, 405, 488, 561, and 637 nm) with 182 detector channels. (B) Spillover spreading matrix calculated in FlowJo V10.10.0 from single stains. (C) Titration of antibodies in human PBMCs. The viability dye Zombie NIR and CD73-biotin/Qdot 525 streptavidin were independently optimized (see for final volumes).

    Journal: STAR Protocols

    Article Title: Protocol for generating high-quality, 45-color spectral flow cytometry data for unsupervised clustering to investigate aging in human PBMCs

    doi: 10.1016/j.xpro.2026.104380

    Figure Lengend Snippet: Optimization of a 45-color spectral panel (A) “Spectral Viewer” overview of all 45 fluorescence spectra acquired using a six-laser–configured ID7000 (320, 355, 405, 488, 561, and 637 nm) with 182 detector channels. (B) Spillover spreading matrix calculated in FlowJo V10.10.0 from single stains. (C) Titration of antibodies in human PBMCs. The viability dye Zombie NIR and CD73-biotin/Qdot 525 streptavidin were independently optimized (see for final volumes).

    Article Snippet: ID7000 Software V2.1 , Sony , https://www.sonybiotechnology.com/instruments/id7000-spectral-cell-analyzer/software.

    Techniques: Fluorescence, Titration

    Autofluorescence (AF) unmixing workflow for the 45-color panel (A) Ribbon plot showing the fluorescence spectrum of unstained PBMCs. Autofluorescence is especially excited by the 320 nm and 355 nm lasers. (B) In the ID7000 Software, the “Autofluorescence Finder” tool is employed to identify and unmix the AF of two distinct populations within the AF root gate, excited by the 320 nm and 355 nm lasers. (C) AF-A and AF-B are highlighted in the “Spectral Reference Viewer” among the 45-color + 2 AF spectra. The fluorochromes with the greatest AF overlap are BUV496 (turquoise) and SparkUV 387 (purple). (D) AF unmixing improves population separation by extracting AF contributions to the negative population for CD28-BUV496 and CD14-SparkUV 387.

    Journal: STAR Protocols

    Article Title: Protocol for generating high-quality, 45-color spectral flow cytometry data for unsupervised clustering to investigate aging in human PBMCs

    doi: 10.1016/j.xpro.2026.104380

    Figure Lengend Snippet: Autofluorescence (AF) unmixing workflow for the 45-color panel (A) Ribbon plot showing the fluorescence spectrum of unstained PBMCs. Autofluorescence is especially excited by the 320 nm and 355 nm lasers. (B) In the ID7000 Software, the “Autofluorescence Finder” tool is employed to identify and unmix the AF of two distinct populations within the AF root gate, excited by the 320 nm and 355 nm lasers. (C) AF-A and AF-B are highlighted in the “Spectral Reference Viewer” among the 45-color + 2 AF spectra. The fluorochromes with the greatest AF overlap are BUV496 (turquoise) and SparkUV 387 (purple). (D) AF unmixing improves population separation by extracting AF contributions to the negative population for CD28-BUV496 and CD14-SparkUV 387.

    Article Snippet: ID7000 Software V2.1 , Sony , https://www.sonybiotechnology.com/instruments/id7000-spectral-cell-analyzer/software.

    Techniques: Fluorescence, Software